Centrifuge-Free Sperm Preparation in an Intelligent Automated In Vitro Fertilization and Intracytoplasmic Sperm Injection Platform

Written By Jen Montoya

US 12,478,405 B2

A method of artificial-intelligence-based robotic pipetting for spermatozoa preparation includes positioning a vessel containing a semen sample within a staging mechanism. The method includes using a robotic pipettor to make at least two droplets within a dish on the staging mechanism. The method includes using the robotic pipettor to connect the at least two droplets with a medium channel according to a programmable design commanded by an artificial intelligence/machine learning system (AI/ML system). The method includes using the robotic pipettor to deposit a quantity of sperm from the semen sample into one of the droplets. The method includes using the AI/ML system to optically scan the quantity of sperm to produce first and second image objects using an imaging system that includes a microscopy system, a camera system, and a lighting system. Creation of the image objects is separated by a specified time duration.

23 Claims, 74 Drawing Sheets

Jen Montoya https://www.brandrxco.com
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