Autonomous Denudation in an Intelligent Automated in Vitro Fertilization and Intracytoplasmic Sperm Injection Platform

Written By Jen Montoya

Flores-Saiffe Farias et al. | Patent No. US 12,349,940 B2

A method for automated, artificial-intelligence-based denu- dation includes scanning, robotically, a dish containing a cumulus-oocyte-complex (COC) using an imaging system and an artificial intelligence/machine learning system (AI/ ML system) to create an image object. The imaging system includes a microscopy system, a camera system, and a lighting system. The dish includes an enzyme to remove cumulus cells from an oocyte among the COC. The method includes identifying the COC within the image object, and a location, based on comparing the image object to a threshold using the AI/ML system. The threshold is an optical pattern with a probability of corresponding to a COC mass. The method includes instructing a robotic pipettor to collect the COC at the location. The method includes iteratively collecting the COC within the robotic pipettor and expelling the COC to return it to the dish until the AI/ML system confirms sufficient removal of cumulus and corona cells.

20 Claims, 74 Drawing

 
Jen Montoya https://www.brandrxco.com
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Egg Preparation in an Intelligent Automated In Vitro Fertilization and Intracytoplasmic Sperm Injection Platform