A Novel 3D-Printed Incubator for Holding Gametes and Embryos During In Vitro Fertilization (IVF) Procedures

Matia Algué et al., 2025


During IVF, embryos need to be temporarily removed from their primary incubators for various manipulations. Secondary benchtop incubators can be used to temporarily house them, reducing stress. 

This study introduces and evaluates a 3D-printed incubator featuring a specially designed lid with channels for uniform gas distribution and magnetic closures for secure sealing.

Testing with mouse embryos demonstrated that the 3D-printed incubator performed identically to standard commercial incubators, with no differences in embryo development, survival rates, or cell counts. The device maintained stable pH levels for 24 hours and recovered quickly from temperature disruptions, proving its reliability for short-term embryo culture during laboratory procedures.

This innovation offers a reliable and cost-effective approach for use in either traditional or automated IVF laboratories.

 
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Validation of a Novel High-Capacity Automated Incubator for In Vitro Fertilization (IVF)